Article
Targeted nanopore sequencing with Cas9-guided adapter ligation.
Nature biotechnology - 1 Apr 2020
Gilpatrick Timothy, Lee Isac, Graham James E, Raimondeau Etienne, Bowen Rebecca, Heron Andrew, Downs Bradley, Sukumar Saraswati, Sedlazeck Fritz J, Timp Winston
Abstract excerpt
Despite recent improvements in sequencing methods, there remains a need for assays that provide high sequencing depth and comprehensive variant detection. Current methods1-4 are limited by the loss of native modifications, short read length, high input requirements, low yield or long protocols. In the present study, we describe nanopore Cas9-targeted sequencing (nCATS), an enrichment strategy that uses targeted...
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