MAGNET-seq: A tandem PCR and hybrid capture method for enhanced target enrichment.

Abstract excerpt

Hybrid-capture based target enrichment and multiplex PCR methods enhance sequencing efficiency by focusing on specific genomic regions, while struggling to enrich tens of regions spanning hundreds to thousands of base pairs. We developed MAGNET-seq (Multiplex Amplification and tarGeted eNrichment of sElecTed sequences), a streamlined method that integrates targeted multiplex PCR with hybrid capture. We evaluated...

Topics

• Humans
• Multiplex Polymerase Chain Reaction
• Mutation
• High-Throughput Nucleotide Sequencing
• DNA Primers
• ErbB Receptors
• Gene Frequency