Sequencing small genomic targets with high efficiency and extreme accuracy.

doi:10.1038/nmeth.3351

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Article

Sequencing small genomic targets with high efficiency and extreme accuracy.

Nature methods - 1 May 2015

Schmitt Michael W, Fox Edward J, Prindle Marc J, Reid-Bayliss Kate S, True Lawrence D, Radich Jerald P, Loeb Lawrence A

Abstract excerpt

The detection of minority variants in mixed samples requires methods for enrichment and accurate sequencing of small genomic intervals. We describe an efficient approach based on sequential rounds of hybridization with biotinylated oligonucleotides that enables more than 1-million-fold enrichment of genomic regions of interest. In conjunction with error-correcting double-stranded molecular tags, our approach...

Topics

Base Sequence
DNA
Drug Resistance, Neoplasm
Gene Expression Regulation, Neoplastic
Genetic Loci
Genetic Variation
Genomics
Humans
Leukemia, Myeloid
Mutation
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA

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Identifiers

PMID: 25849638
DOI: 10.1038/nmeth.3351
PMCID: PMC4414912

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