Article

Nanopore sequencing for N1-methylpseudouridine in RNA reveals sequence-dependent discrimination of the modified nucleotide triphosphate during transcription

2022-06-03

Abstract excerpt

Direct RNA sequencing with a commercial nanopore platform was used to sequence RNA containing uridine (U), pseudouridine (Ψ), or N1-methylpseudouridine (m 1 Ψ) generated by in vitro transcription (IVT). The base calling data as well as the ionic currents and dwell times for U, Ψ, or m 1 Ψ as they translocated through the helicase and nanopore proteins identified diagnostic signatures for Ψ and m 1 Ψ; however, t...

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Literature Corpus work: 6e5f9a4a-61b2-5f23-8c7d-cb61b36e522e
DOI: 10.1101/2022.06.03.494690

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