Article

Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct and long-read sequencing

2021-11-04

Abstract excerpt

We developed and applied a semi-quantitative method for high-confidence identification of pseudouridylated sites on mammalian mRNAs via direct long-read nanopore sequencing. A comparative analysis of a modification-free transcriptome reveals that the depth of coverage and specific k-mer sequences are critical parameters for accurate basecalling. By adjusting these parameters for high-confidence U-to-C basecalling...

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Literature Corpus work: f7392d02-fa35-557d-85bb-9fcd9dd15601
DOI: 10.1101/2021.11.03.467190

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