Article

RNA folding below the nanopore impairs direct RNA sequencing fidelity and modification calling

2026-07-13

Abstract excerpt

<title>Abstract</title> <p>RNA modifications are key for biological processes. Oxford Nanopore direct RNA sequencing (DRS) infers modifications from subtle ionic-current differences, but these signals are prone to misinterpretation due to systematic distortions. Here we identify an unrecognized source of DRS errors: RNA secondary structure folding below the nanopore during sequencing. Across engineered transcript...

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Literature Corpus work: ebcbdaf5-a58c-551b-8291-5cc21d8001cd
DOI: 10.21203/rs.3.rs-10315656/v1

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