Article

Messenger-RNA Modification Standards and Machine Learning Models Facilitate Absolute Site-Specific Pseudouridine Quantification

2022-05-06

Abstract excerpt

Enzyme-mediated chemical modifications to mRNA are important for fine-tuning gene expression, but they are challenging to quantify due to low copy number and limited tools for accurate detection. Existing studies have typically focused on the identification and impact of adenine modifications on mRNA (m 6 A and inosine) due to the availability of analytical methods. The pseudouridine (Ψ) mRNA modification is also...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 137b6678-b7af-5225-84d7-9a94531c0fc6
DOI: 10.1101/2022.05.06.490948

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Messenger-RNA Modification Standards and Machine Learning Models Facilitate Absolute Site-Specific Pseudouridine QuantificationDOI 10.1101/2022.05.06.490948Read

Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct and long-read sequencingSemantic proximity Read

Enzyme-mediated alkynylation enables transcriptome-wide identification of pseudouridine modificationsSemantic proximity Read

Quantitative profiling of pseudouridylation landscape in the human transcriptomeSemantic proximity Read

A Radiolabeling-Free, qPCR-Based Method for Locus-Specific Pseudouridine Detection.Semantic proximity Read

Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolutionSemantic proximity Read

Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptomeSemantic proximity Read

ModiDeC: a multi-RNA modification classifier for direct nanopore sequencingSemantic proximity Read

Promoted Read-through and Mutation Against Pseudouridine-CMC by an Evolved Reverse TranscriptaseSemantic proximity Read

Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based techniqueSemantic proximity Read

Recent developments, opportunities, and challenges in the study of mRNA pseudouridylationSemantic proximity Read

Direct RNA nanopore sequencing reveals rapid RNA modification changes following glucose stimulation of human pancreatic beta-cell linesSemantic proximity Read

Direct RNA sequencing enables improved transcriptome assessment and tracking of RNA modifications for medical applicationsSemantic proximity Read