Article

CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary cells

2020-04-18

Abstract excerpt

<h4>ABSTRACT</h4> Base editors allow for precise nucleotide editing without the need for genotoxic double-stranded breaks. Prior work has used base editors to knockout genes by introducing premature stop codons or by disrupting conserved splice-sites, but no direct comparison exists between these methods. Additionally, while base editor mediated disruption of splice sites has been used to shift the functional iso...

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Literature Corpus work: 6cf2464e-d8d5-5d45-8536-797d7fc4887b
DOI: 10.1101/2020.04.16.045336

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