Article
Optimized base editors enable efficient editing in cells, organoids and mice.
Nature biotechnology - 1 Oct 2018
Zafra Maria Paz, Schatoff Emma M, Katti Alyna, Foronda Miguel, Breinig Marco, Schweitzer Anabel Y, Simon Amber, Han Teng, Goswami Sukanya, Montgomery Emma, Thibado Jordana, Kastenhuber Edward R, Sánchez-Rivera Francisco J, Shi Junwei, Vakoc Christopher R, Lowe Scott W, Tschaharganeh Darjus F, Dow Lukas E
Abstract excerpt
CRISPR base editing enables the creation of targeted single-base conversions without generating double-stranded breaks. However, the efficiency of current base editors is very low in many cell types. We reengineered the sequences of BE3, BE4Gam, and xBE3 by codon optimization and incorporation of additional nuclear-localization sequences. Our collection of optimized constitutive and inducible base-editing vector...
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