Article
Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations.
Nature cell biology - 1 May 2021
Wang Lijie, Xue Wei, Zhang Hongxia, Gao Runze, Qiu Houyuan, Wei Jia, Zhou Lina, Lei Yun-Ni, Wu Xiaocheng, Li Xiao, Liu Chengfang, Wu Jing, Chen Qiubing, Ma Hanhui, Huang Xingxu, Cai Cheguo, Zhang Ying, Yang Bei, Yin Hao, Yang Li, Chen Jia
Abstract excerpt
The fusion of CRISPR-Cas9 with cytidine deaminases leads to base editors (BEs) capable of programmable C-to-T editing, which has potential in clinical applications but suffers from off-target (OT) mutations. Here, we used a cleavable deoxycytidine deaminase inhibitor (dCDI) domain to construct a transformer BE (tBE) system that induces efficient editing with only background levels of genome-wide and...
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