Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs

Abstract excerpt

<title>Abstract</title> <p>Canonical CRISPR screens rely on Cas9-induced DNA double-strand breaks (DSBs) to generate targeted gene knockouts. These DSB-dependent methodologies may yield false-positive results by mistakenly assuming targeted loci as essential for cell viability, especially when high-copy-number sites are targeted. Here, we use CRISPR cytosine base editors for genome-scale knockout screens by pertu...

Topics

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• BRCA1 Protein
• Breast Neoplasms
• Cell Line, Tumor
• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Associated Protein 9
• Gene Editing
• High-Throughput Nucleotide Sequencing
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

878e51b1-a59b-50f7-8ef1-e7ace23ef34c

DOI

10.21203/rs.3.rs-57831/v1