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Article

Design and application of the transformer base editor in mammalian cells and mice.

Nature protocols - 1 Nov 2023

Han Wenyan, Gao Bao-Qing, Zhu Junjie, He Zongxing, Li Jianfeng, Yang Li, Chen Jia

Abstract excerpt

Fusing apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like cytidine deaminase with catalytically impaired Cas proteins (e.g., nCas9 or dCas9) provides a novel gene-editing technology, base editing, that grants targeted base substitutions with high efficiency. However, genome-wide and transcriptome-wide off-target mutations are observed in base editing, which raises safety concerns regarding...

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PMID: 37794072
DOI: 10.1038/s41596-023-00877-w

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