Article

Modelling clinical DNA fragmentation in the development of universal PCR-based assays for bisulfite, FFPE and cfDNA sample analysis

2022-06-22

Abstract excerpt

In fragmented DNA, PCR-based methods quantify the number of intact regions at a specific amplicon length. However, the relationship between the population of DNA fragments within a sample and the likelihood they will amplify has not been fully described. To address this, we have derived a mathematical equation that relates the distribution profile of a stochastically fragmented DNA sample to the probability that a...

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Identifiers and source

Literature Corpus work: 6af37062-1bd0-5429-b69a-706f93a64228
DOI: 10.21203/rs.3.rs-1728412/v1

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