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Adopting Duplex Sequencing™ Technology for Genetic Toxicity Testing: A Proof-of-Concept Mutagenesis Experiment with N-Ethyl-N-Nitrosourea (ENU)-Exposed Rats

2023-05-09

Abstract excerpt

<h4>ABSTRACT</h4> Duplex sequencing (DuplexSeq) is an error-corrected next-generation sequencing (ecNGS) method in which molecular barcodes informatically link PCR-copies back to their source DNA strands, enabling computational removal of errors by comparing grouped strand sequencing reads. The resulting background of less than one artifactual mutation per 10 7 nucleotides allows for direct detection of somatic...

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Literature Corpus work: 6248cf8d-1437-5c58-a5f1-eb661141aa9b
DOI: 10.1101/2023.05.08.539833

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