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Article

Alignment between Duplex Sequencing and transgenic rodent mutation assay data in the assessment of in vivo NDMA-induced mutagenesis.

Archives of toxicology - 1 Oct 2025

Ashford Anne L, Nachmanson Daniela, Wills John W, Higgins Jacob E, Smith Thomas H, Vavra Kevin C, Dahalan Farah A, Howe Jonathan, Elloway Joanne M, Salk Jesse J, Doherty Ann, Lynch Anthony M

Abstract excerpt

The nitrosamine N-nitrosodimethylamine (NDMA) is a mutagen and rodent carcinogen that has been identified as a process impurity in some commercially available medicines, leading to market withdrawals and new impurity control measures. Error-corrected DNA sequencing techniques, such as Duplex Sequencing (DS), have error rates low enough to revolutionise genetic toxicology testing by directly measuring in vivo...

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Identifiers

PMID: 40676325
DOI: 10.1007/s00204-025-04121-0
PMCID: PMC12454481

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