Article

Optimized Calcium-Phosphate based co-transfection of TAU and tdTomato into human iPSC-derived neurons for the study of intracellular distribution of wildtype and mutant human TAU.

2023-06-08

Abstract excerpt

<title>Abstract</title> The study of TAU protein in disease-relevant neuronal cells in culture requires efficient delivery systems for transfection of exogenous TAU and also modulators and interactions of TAU. Transfection of cultivated cells using calcium phosphate precipitation is a simple and cost-effective approach, also for difficult-to-transfect and sensitive cells such as primary neurons. Because of its...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 60256fb3-0083-5fb6-8943-b122a1bd44fd
DOI: 10.21203/rs.3.pex-2142/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Optimized Calcium-Phosphate based co-transfection of TAU and tdTomato into human iPSC-derived neurons for the study of intracellular distribution of wildtype and mutant human TAU.DOI 10.21203/rs.3.pex-2142/v1Read

An update to: Tracking TAU in neurons: How to transfect and track exogenous TAU in primary neuronsSemantic proximity Read

Cultivation, differentiation, and lentiviral transduction of human induced pluripotent stem cell (hiPSC)-derived glutamatergic neurons for studying human TAUSemantic proximity Read

Calcium dysregulation contributes to neurodegeneration in FTLD patient iPSC-derived neuronsSemantic proximity Read

Short-Chain Polyphosphates Induce Tau Fibrillation and Neurotoxicity in Human iPSC-Derived Retinal NeuronsSemantic proximity Read

Tau seeds translocate across the cell membrane to initiate aggregationSemantic proximity Read

MAPT genotype-dependent mitochondrial aberration and ROS production trigger dysfunction and death in cortical neurons of patients with hereditary FTLD.Semantic proximity Read

Tau inhibits mitochondrial calcium efflux and makes neurons vulnerable to calcium-induced cell death.Semantic proximity Read

IPSC-derived midbrain astrocytes from Parkinson’s disease patients carrying pathogenic SNCA mutations exhibit alpha-synuclein aggregation, mitochondrial fragmentation and excess calcium releaseSemantic proximity Read

Embryonic Stem Cell–Based Modeling of Tau Pathology in Human NeuronsSemantic proximity Read

Intraneuronal tau aggregation induces the integrated stress response in astrocytesSemantic proximity Read

deCLUTTER 2+ pipeline to analyze calcium traces in a novel stem cell model for ventral midbrain patterned astrocytesSemantic proximity Read

Twelve phosphomimetic mutations induce the assembly of recombinant full-length human tau into paired helical filaments.Semantic proximity Read