Article
Detect-seq reveals out-of-protospacer editing and target-strand editing by cytosine base editors.
Nature methods - 1 Jun 2021
Lei Zhixin, Meng Haowei, Lv Zhicong, Liu Menghao, Zhao Huanan, Wu Hao, Zhang Xiaoxue, Liu Lulu, Zhuang Yuan, Yin Kailin, Yan Yongchang, Yi Chengqi
Abstract excerpt
Cytosine base editors (CBEs) have the potential to correct human pathogenic point mutations. However, their genome-wide specificity remains poorly understood. Here we report Detect-seq for the evaluation of CBE specificity. It enables sensitive detection of CBE-induced off-target sites at the genome-wide level. Detect-seq leverages chemical labeling and biotin pulldown to trace the editing intermediate...
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