Article

Diversified, miniaturized and ancestral parts for mammalian genome engineering and molecular recording

2024-10-01

Abstract excerpt

As the synthetic biology and genome engineering fields mature and converge, there is a clear need for a “parts list” of components that are diversified with respect to both functional activity (to facilitate design) and primary sequence (to facilitate assembly). Here we designed libraries composed of extant, ancestral, mutagenized or miniaturized variants of Pol III promoters or guide RNA (gRNA) scaffolds and quan...

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Identifiers and source

Literature Corpus work: 5ead069b-77a8-563c-a296-c0c0a466a406
DOI: 10.1101/2024.09.30.615957

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