Article

Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomato

2019-01-16

Abstract excerpt

<h4>ABSTRACT</h4> Genome editing via the homology-directed repair (HDR) pathway in somatic plant cells is very inefficient compared to error-prone repair by nonhomologous end joining (NHEJ). Here, we increased HDR-based genome editing efficiency approximately 3-fold compared to a Cas9-based single-replicon system via the use of de novo multi-replicon systems equipped with CRISPR/LbCpf1 in tomato and obtained rep...

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Literature Corpus work: 5dea2af0-b326-5343-a123-6f7e41defae7
DOI: 10.1101/521419

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