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Article

Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells.

Scientific reports - 24 Feb 2024

Slaman Ellen, Kottenhagen Lisanne, de Martines William, Angenent Gerco C, de Maagd Ruud A

Abstract excerpt

Cas12a is a promising addition to the CRISPR toolbox, offering versatility due to its TTTV-protospacer adjacent motif (PAM) and the fact that it induces double-stranded breaks (DSBs) with single-stranded overhangs. We characterized Cas12a-mediated genome editing in tomato using high-throughput amplicon sequencing on protoplasts. Of the three tested variants, Lachnospiraceae (Lb) Cas12a was the most efficient....

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Identifiers

PMID: 38402312
DOI: 10.1038/s41598-024-55088-4
PMCID: PMC10894265

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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