Article

Impact of temperature and time on DNA-free Cas9-ribonucleoprotein mediated gene editing in wheat protoplasts and immature embryos

2022-04-06

Abstract excerpt

<h4>Summary</h4> The advancement of precision engineering for crop trait improvement is important in the face of rapid population growth, climate change, and disease. To this end, targeted double-stranded break technology using RNA-guided Cas9 has been adopted widely for genome editing in plants. Agrobacterium or particle bombardment-based delivery of plasmids encoding Cas9 and guide RNA (gRNA) is common, but re...

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Literature Corpus work: 835acb47-1009-5232-a0e5-ecaa35b21c7e
DOI: 10.1101/2022.04.05.487229

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