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Article

Efficient in planta gene targeting in tomato using geminiviral replicons and the CRISPR/Cas9 system.

The Plant journal : for cell and molecular biology - 1 Jul 2018

Dahan-Meir Tal, Filler-Hayut Shdema, Melamed-Bessudo Cathy, Bocobza Samuel, Czosnek Henryk, Aharoni Asaph, Levy Avraham A

Abstract excerpt

Current breeding relies mostly on random mutagenesis and recombination to generate novel genetic variation. However, targeted genome editing is becoming an increasingly important tool for precise plant breeding. Using the CRISPR-Cas system combined with the bean yellow dwarf virus rolling circle replicon, we optimized a method for targeted mutagenesis and gene replacement in tomato. The carotenoid isomerase...

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PMID: 29668111
DOI: 10.1111/tpj.13932

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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