Article

A dual sgRNA approach for functional genomics in <i>Arabidopsis thaliana</i> <sup>[OPEN]</sup>

2017-08-09

Abstract excerpt

Reverse genetics uses loss-of-function alleles to interrogate gene function. The advent of CRISPR/Cas9-based gene editing now allows to generate knock-out alleles for any gene and entire gene families. Even in the model plant Arabidopsis thaliana , gene editing is welcomed as T-DNA insertion lines do not always generate null alleles. Here, we show efficient generation of heritable mutations in Arabidopsis using C...

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Literature Corpus work: 696d7e18-7c6d-555c-a94d-34fea0954e09
DOI: 10.1101/172676

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