Establishment of an efficient and PAM-relaxed LbCas12a genome editing tool in plants.

doi:10.1111/nph.71498

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Establishment of an efficient and PAM-relaxed LbCas12a genome editing tool in plants.

The New phytologist - 1 Oct 2026

Wang Yaolong, Xu Huanhuan, Lu Wenjun, Wan Zhiwen, Xu Ziyan, Wang Wenlong, Chen Chengyu, Pan Eryang, Jiang Fangling, Liu Tongkun, Li Ying, Xiao Dong, Yang Xuedong, Li Fangfang, Hou Xilin, Zhang Changwei

Abstract excerpt

Cas12a is widely used in plant genome editing, but its targeting scope is constrained by stringent protospacer adjacent motif (PAM) requirements and variable activity across species, limiting its application at diverse genomic loci. LbCas12a-RRV-based editing system was established in nonheading Chinese cabbage, and T5exo-PF-LbCas12a was generated by introducing a triple mutation (D535G/S551F/D665N) and fusing...

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Topics

Genome, Plant
Oryza
Solanum lycopersicum
Brassica
Base Sequence
Nucleotide Motifs

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Mutation

CRISPR-Cas Systems

Plants, Genetically Modified

Genetic Engineering

Gene Editing

CRISPR-Associated Proteins

Bacterial Proteins

Endodeoxyribonucleases

Source

Open in PubMed Open full text

Identifiers

PMID: 42552655
DOI: 10.1111/nph.71498
PMCID: PMC13583368

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