Virus-induced transgene- and tissue culture-free heritable genome editing in tomato

Abstract excerpt

Genome editing has emerged as a powerful tool for genome manipulation and trait improvement in crops. However, most commonly used approaches rely on tissue culture and transgenic materials, which are time-consuming, labor-intensive, and often strongly genotype-dependent. Here, we developed a tobacco rattle virus (TRV)–based system to deliver the compact ISYmu1 TnpB endonuclease, coupled with in planta shoot regen...

Topics

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• CRISPR-Cas Systems
• Gene Editing
• Genome, Plant
• Genotype
• Plant Breeding
• Plants
• Plants, Genetically Modified
• RNA Viruses

Identifiers and source

Literature Corpus work

ed62ee9c-6a52-50ee-ab98-0d1dc7dab7c3

DOI

10.1101/2025.11.08.687402