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Article

An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design.

Scientific reports - 1 Apr 2016

Zhao Yongping, Zhang Congsheng, Liu Wenwen, Gao Wei, Liu Changlin, Song Gaoyuan, Li Wen-Xue, Mao Long, Chen Beijiu, Xu Yunbi, Li Xinhai, Xie Chuanxiao

Abstract excerpt

Precision DNA/gene replacement is a promising genome-editing tool that is highly desirable for molecular engineering and breeding by design. Although the CRISPR/Cas9 system works well as a tool for gene knockout in plants, gene replacement has rarely been reported. Towards this end, we first designed a combinatory dual-sgRNA/Cas9 vector (construct #1) that successfully deleted miRNA gene regions (MIR169a and...

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Identifiers

PMID: 27033976
DOI: 10.1038/srep23890
PMCID: PMC4817149

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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