Article

High-fidelity CRISPR genome editing of single-nucleotide mutation with near-complementary guide RNA via enhanced target binding kinetics

2025-09-04

Abstract excerpt

The CRISPR-Cas9 system is a powerful genome editing tool capable of precisely recognizing and cleaving specific DNA sequences, and has been extensively investigated as a strategy for correcting mutations associated with genetic diseases and cancer. However, conventional CRISPR genome engineering often fail to discriminate single-nucleotide mutations from wild-type alleles when the mutation is located outside the p...

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Literature Corpus work: 5d00e7b2-88ec-52b1-9e13-ac420e14697e
DOI: 10.1101/2025.09.02.671676

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