Article
Enhancement of target specificity of CRISPR-Cas12a by using a chimeric DNA-RNA guide.
Nucleic acids research - 4 Sept 2020
Kim Hanseop, Lee Wi-Jae, Oh Yeounsun, Kang Seung-Hun, Hur Junho K, Lee Hyomin, Song WooJeung, Lim Kyung-Seob, Park Young-Ho, Song Bong-Seok, Jin Yeung Bae, Jun Bong-Hyun, Jung Cheulhee, Lee Dong-Seok, Kim Sun-Uk, Lee Seung Hwan
Abstract excerpt
The CRISPR-Cas9 system is widely used for target-specific genome engineering. CRISPR-Cas12a (Cpf1) is one of the CRISPR effectors that controls target genes by recognizing thymine-rich protospacer adjacent motif (PAM) sequences. Cas12a has a higher sensitivity to mismatches in the guide RNA than does Cas9; therefore, off-target sequence recognition and cleavage are lower. However, it tolerates mismatches in...
Topics
- Bacterial Proteins
- Base Pair Mismatch
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- DNA
- DNA Cleavage
- Endodeoxyribonucleases
- Gene Editing
- Humans
- Models, Molecular