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Article

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity.

Nature communications - 13 Apr 2018

Cromwell Christopher R, Sung Keewon, Park Jinho, Krysler Amanda R, Jovel Juan, Kim Seong Keun, Hubbard Basil P

Abstract excerpt

Off-target DNA cleavage is a paramount concern when applying CRISPR-Cas9 gene-editing technology to functional genetics and human therapeutic applications. Here, we show that incorporation of next-generation bridged nucleic acids (2',4'-BNANC[N-Me]) as well as locked nucleic acids (LNA) at specific locations in CRISPR-RNAs (crRNAs) broadly reduces off-target DNA cleavage by Cas9 in vitro and in cells by several...

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Identifiers

PMID: 29654299
DOI: 10.1038/s41467-018-03927-0
PMCID: PMC5899152

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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