Article
Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity.
Nature communications - 25 Jan 2022
Fu Rongjie, He Wei, Dou Jinzhuang, Villarreal Oscar D, Bedford Ella, Wang Helen, Hou Connie, Zhang Liang, Wang Yalong, Ma Dacheng, Chen Yiwen, Gao Xue, Depken Martin, Xu Han
Abstract excerpt
The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (gRNA) and the targeted DNA, yet the sequence-dependent rules underlying off-target effects are not fully understood. To systematically explore the sequence determinants governing CRISPR/Cas9 specificity, here we describe a dual-target system to measure the relative cleavage rate between off- and on-target sequences...
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