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Article

Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1.

Molecular cell - 6 Jul 2017

Nishimasu Hiroshi, Yamano Takashi, Gao Linyi, Zhang Feng, Ishitani Ryuichiro, Nureki Osamu

Abstract excerpt

The RNA-guided Cpf1 nuclease cleaves double-stranded DNA targets complementary to the CRISPR RNA (crRNA), and it has been harnessed for genome editing technologies. Recently, Acidaminococcus sp. BV3L6 (AsCpf1) was engineered to recognize altered DNA sequences as the protospacer adjacent motif (PAM), thereby expanding the target range of Cpf1-mediated genome editing. Whereas wild-type AsCpf1 recognizes the TTTV...

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Identifiers

PMID: 28595896
DOI: 10.1016/j.molcel.2017.04.019
PMCID: PMC5957533

Genes

PAM

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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