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Article

Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases.

Nucleic acids research - 17 Apr 2020

Tóth Eszter, Varga Éva, Kulcsár Péter István, Kocsis-Jutka Virág, Krausz Sarah Laura, Nyeste Antal, Welker Zsombor, Huszár Krisztina, Ligeti Zoltán, Tálas András, Welker Ervin

Abstract excerpt

The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs that are active in mammalian and plant cells, by combining the mutations of their corresponding RR and RVR variants...

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Identifiers

PMID: 32107556
DOI: 10.1093/nar/gkaa110
PMCID: PMC7144938

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