Article

A CRISPR/Cas9-based system using dual-sgRNAs for efficient gene deletion in <i>Mycobacterium abscessus</i>

2025-05-29

Abstract excerpt

The increasing global prevalence of Mycobacterium abscessus infections presents a significant clinical challenge due to the pathogen’s intrinsic resistance to multiple antibiotics and poor treatment outcomes. Despite the necessity of genetic tools for studying its physiology, pathogenesis, and drug resistance, efficient methods for large-fragment deletions remain underdeveloped. Here, we report a CRISPR/Cas9-base...

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Literature Corpus work: 568e76bb-81a4-5e53-b483-fe8eff2682b4
DOI: 10.1101/2025.05.28.656731

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