Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CRISPR-Cas12a assisted precise genome editing of Mycolicibacterium neoaurum

13 Oct 2021

Abstract excerpt

Efficient and convenient genetic manipulation of mycobacteria, important microorganisms in human healthcare and the pharmaceutical industry, is limited. In this study, using a model strain Mycolicibacterium neoaurum ATCC 25795, the classical bacterium for the production of valuable steroidal pharmaceuticals, a genome editing system employing CRISPR-Cas12a to achieve efficient and precise genetic manipulation has...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR and Genetic Engineering
Share to Insect symbiosis and bacterial influences
Share to Mycobacterium research and diagnosis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 34653700
DOI: 10.1016/j.nbt.2021.10.003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas12a assisted precise genome editing of Mycolicibacterium neoaurumPMID 34653700Read ↗

Efficient genome editing in pathogenic mycobacteria using Streptococcus thermophilus CRISPR1-Cas9Semantic proximity Read ↗

The CRISPR toolbox in medical mycology: State of the art and perspectivesSemantic proximity Read ↗

A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium abscessusSemantic proximity Read ↗

ERA-CRISPR/Cas12a system: a rapid, highly sensitive and specific assay for Mycobacterium tuberculosisSemantic proximity Read ↗

The future of CRISPR in Mycobacterium tuberculosis infectionSemantic proximity Read ↗

CRISPR-Cas12a-Assisted Recombineering in Bacteria.Semantic proximity Read ↗

Genetic Engineering in Bacteria, Fungi, and OomycetesSemantic proximity Read ↗

A CRISPR/Cas9-based system using dual-sgRNAs for efficient gene deletion in <i>Mycobacterium abscessus</i>Semantic proximity Read ↗

A CRISPR-Nonhomologous End-Joining-based strategy for rapid and efficient gene disruption in <i>Mycobacterium abscessus</i>Semantic proximity Read ↗

Advances and Challenges in CRISPR/Cas-Based Fungal Genome Engineering for Secondary Metabolite Production: A ReviewSemantic proximity Read ↗

Reprogramming the endogenous type III-A CRISPR-Cas system for genome editing, RNA interference and CRISPRi screening in <i>Mycobacterium tuberculosis</i>Semantic proximity Read ↗

Reprogramming Mycobacterium Tuberculosis CRISPR System for Gene Editing and Genome-Wide RNA Interference ScreeningSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.