Efficient genome editing in pathogenic mycobacteria using Streptococcus thermophilus CRISPR1-Cas9

Abstract excerpt

The ability to genetically engineer pathogenic mycobacteria has increased significantly over the last decades due to the generation of new molecular tools. Recently, the application of the Streptococcus pyogenes and the Streptococcus thermophilus CRISPR-Cas9 systems in mycobacteria has enabled gene editing and efficient CRISPR interference-mediated transcriptional regulation. Here, we converted CRISPR...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to CRISPR and Genetic Engineering
• Share to Mycobacterium research and diagnosis
• Share to RNA and protein synthesis mechanisms