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Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing

11 Nov 2021

Abstract excerpt

A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read and long-read (nanopore) sequencing together with a new computational pipeline (FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation detection in single cells. We identify thousands of unannotated isoforms and find conserved functional modules that are enriched for alternative transcript usage in...

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PMID: 34763716
DOI: 10.1186/s13059-021-02525-6

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