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Article

High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors.

Nature communications - 11 Jul 2023

Shiau Cheng-Kai, Lu Lina, Kieser Rachel, Fukumura Kazutaka, Pan Timothy, Lin Hsiao-Yun, Yang Jie, Tong Eric L, Lee GaHyun, Yan Yuanqing, Huse Jason T, Gao Ruli

Abstract excerpt

Single-cell nanopore sequencing of full-length mRNAs transforms single-cell multi-omics studies. However, challenges include high sequencing errors and dependence on short-reads and/or barcode whitelists. To address these, we develop scNanoGPS to calculate same-cell genotypes (mutations) and phenotypes (gene/isoform expressions) without short-read nor whitelist guidance. We apply scNanoGPS onto 23,587 long-read...

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PMID: 37433798
DOI: 10.1038/s41467-023-39813-7
PMCID: PMC10336110

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