Article

Comprehensive characterization of single cell full-length isoforms in human and mouse with long-read sequencing

2020-08-10

Abstract excerpt

Alternative splicing shapes the phenotype of cells in development and disease. Long-read RNA-sequencing recovers full-length transcripts but has limited throughput at the single-cell level. Here we developed single-cell full-length transcript sequencing by sampling ( FLT-seq ), together with the computational pipeline FLAMES to overcome these issues and perform isoform discovery and quantification, splicing anal...

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Literature Corpus work: fa46bb2f-6027-5e1c-8780-34633c338e71
DOI: 10.1101/2020.08.10.243543

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