Article
A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Sousounis Konstantinos, Courtemanche Katharine, Whited Jessica L
Abstract excerpt
Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.
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