Article

Isoform-specific single-cell perturb-seq reveals distinct functions of alternative promoters in drug response

2025-07-16

Abstract excerpt

CRISPR interference (CRISPRi) screens have emerged as powerful tools for dissecting gene function, yet their application to genes with multiple promoters, which comprise over 60% of human genes, remains poorly understood. Here, we demonstrate that CRISPR-dCas9-based screens exhibit widespread promoter specificity, with untargeted promoters often showing compensatory upregulation to maintain gene expression. Levera...

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Literature Corpus work: 4e041ac2-c8e7-55c0-ab3c-5dafeb1f4d3c
DOI: 10.1101/2025.07.14.664827

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Isoform-specific single-cell perturb-seq reveals distinct functions of alternative promoters in drug responseDOI 10.1101/2025.07.14.664827Read

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