Article

Phage DNA polymerase prevents deleterious on-target DNA damage and enhances precise CRISPR/Cas9 editing

2023-01-11

Abstract excerpt

Common unintended chromosomal alterations induced by CRISPR/Cas9 in mammalian cells, particularly on-target large deletions and chromosomal translocations present a safety challenge for genome editing. Base editing and prime editing that can precisely introduce desired edits without double-stranded breaks and exogenous DNA templates face their own challenges. Thus, there is still an unmet need to develop safer and...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: aa0647bc-c7fc-5f9b-8c1a-0899612c0e8a
DOI: 10.1101/2023.01.10.523496

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Phage DNA polymerase prevents deleterious on-target DNA damage and enhances precise CRISPR/Cas9 editingDOI 10.1101/2023.01.10.523496Read

Preventing large deletions and chromosome loss in engineered human primary T cells by CasPlus with optimized guide RNAsSemantic proximity Read

CRISPR Therapeutics for Duchenne Muscular DystrophySemantic proximity Read

Making gene editing a therapeutic realitySemantic proximity Read

Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future InnovationsSemantic proximity Read

Massively Targeted Evaluation of Therapeutic CRISPR Off-Targets in CellsSemantic proximity Read

Efficient Homology-Directed Repair with Circular Single-Stranded DNA DonorsSemantic proximity Read

Global detection of DNA repair outcomes induced by CRISPR-Cas9Semantic proximity Read

CRISPR DNA base editors with reduced RNA off-target and self-editing activitiesSemantic proximity Read

Genome-wide structural variant landscape following HDR-enhanced CRISPR editing in human hematopoietic stem and progenitor cellsSemantic proximity Read

Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9.Semantic proximity Read

Methods and techniques enabling multi-kilobase long-range genomic rewrite/replace editingSemantic proximity Read

Gene Editing for Duchenne Muscular Dystrophy: From Experimental Models to Emerging TherapiesSemantic proximity Read