Article

Mutation-specific reporter for the optimization and enrichment of prime editing

2021-05-08

Abstract excerpt

We present a fluorescent prime editing and enrichment reporter (fluoPEER), which can be tailored to any genomic target site. This system rapidly and faithfully ranks the efficiency of prime edit guide RNAs (pegRNAs) and any prime editor protein, including novel variants with flexible PAM recognition. Successful reporter editing enriches for genomic editing. FluoPEER can be employed for efficient correction of pati...

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Literature Corpus work: 498c05fa-e1f2-5670-ab40-f9915b64313b
DOI: 10.1101/2021.05.08.443062

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