Article
PrimeDesign software for rapid and simplified design of prime editing guide RNAs.
Nature communications - 15 Feb 2021
Hsu Jonathan Y, Grünewald Julian, Szalay Regan, Shih Justine, Anzalone Andrew V, Lam Kin Chung, Shen Max W, Petri Karl, Liu David R, Joung J Keith, Pinello Luca
Abstract excerpt
Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation...
Topics
- Base Pairing
- Base Sequence
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Databases, Genetic
- Fabry Disease
- Gene Editing
- Genome, Human
- Green Fluorescent Proteins
- HEK293 Cells
- Hemophilia A