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Article

PrimeDesign software for rapid and simplified design of prime editing guide RNAs.

Nature communications - 15 Feb 2021

Hsu Jonathan Y, Grünewald Julian, Szalay Regan, Shih Justine, Anzalone Andrew V, Lam Kin Chung, Shen Max W, Petri Karl, Liu David R, Joung J Keith, Pinello Luca

Abstract excerpt

Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation...

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Identifiers

PMID: 33589617
DOI: 10.1038/s41467-021-21337-7
PMCID: PMC7884779

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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