Article

Implementation of CRISPR-Cas13a system in fission yeast and its repurposing for precise RNA editing

2018-03-19

Abstract excerpt

In contrast to genome editing that introduces genetic changes at DNA level, disrupting or editing genes’ transcripts provides a distinctive approach to perturb a genetic system, offering benefits complementary to classic genetic approaches. To develop a new toolset for manipulation of RNA, we first implemented a member of type VI CRISPR systems, Cas13a from Leptotrichia shahii (LshCas13a) in Schizosaccharomyces...

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Literature Corpus work: c5ea74da-ea61-54f0-8b38-be0f7bd07b16
DOI: 10.1101/284661

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