Engineering improved Cas13 effectors for targeted post-transcriptional regulation of gene expression

Abstract excerpt

<h4>Summary</h4> Cas13 is a family of unique RNA-targeting CRISPR-Cas effectors, making it an appealing tool for probing and perturbing RNA function. However only a few Cas13 homologs have been shown to mediate robust RNA targeting in human cells, suggesting that unknown elements may be limiting their efficacy. Furthermore, many Cas13 enzymes show high degrees of toxicity upon targeting and have not been shown to...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Associated Proteins
• CRISPR-Cas Systems
• Gene Editing
• HEK293 Cells
• Mutation
• RNA
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

44dd9dc6-7d1c-5dfa-9c3f-2ab3aa45b737

DOI

10.1101/2021.05.26.445687