Article

Efficient and flexible strategies for gene cloning and vector construction using overlap PCR

2017-01-18

Abstract excerpt

Gene cloning and vector construction are basic technologies in modern molecular biology for gene functional study. Here, we present flexible and efficient strategies for gene cloning and vector construction using overlap PCR. We firstly cloned the open reading frames (ORFs) of the porcine MSTN , chicken OVA and human α-glucosidase genes by overlap PCR-based assembling of their exons, which could be amplified wi...

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Literature Corpus work: 4899a32c-b733-5d10-8ac1-7135b5940915
DOI: 10.1101/101147

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