Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis.

doi:10.1155/2016/8041532

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Article

Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis.

BioMed research international - 1 Jan 2016

Hussain Hasnain, Chong Nikson Fatt-Ming

Abstract excerpt

The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method. This combined method allows up to 6 base pairs to be mutated at a time and requires a total of 40-45 PCR cycles. A total of eight mutagenesis experiments were successfully carried out, with...

Topics

Base Pair Mismatch
Codon
DNA
Multiplex Polymerase Chain Reaction
Mutagenesis, Site-Directed
Mutation

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Identifiers

PMID: 27995143
DOI: 10.1155/2016/8041532
PMCID: PMC5138438

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