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Article

Two cat expression vectors for cloning and generation of 3'- and 5'-deletion mutants.

Gene - 2 Jan 1992

Kumar G

Abstract excerpt

The construction of two versatile cat vectors, pGK0CAT and pGKA10CAT, is reported. These vectors possess multiple cloning sites derived from the Bluescript pKS(+) plasmid that allow the cloning of diverse DNA fragments. From a single cloned insert, i.e., a putative promoter element, one can use the exonuclease III (ExoIII) and S1 or mung-bean nuclease method to generate sequential deletion mutants of the 3' and...

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Identifiers

PMID: 1544565
DOI: 10.1016/0378-1119(92)90450-4

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Source receipt: pubmed, retrieved 7 Sept 2026.

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