Article
Two cat expression vectors for cloning and generation of 3'- and 5'-deletion mutants.
Gene - 2 Jan 1992
Kumar G
Abstract excerpt
The construction of two versatile cat vectors, pGK0CAT and pGKA10CAT, is reported. These vectors possess multiple cloning sites derived from the Bluescript pKS(+) plasmid that allow the cloning of diverse DNA fragments. From a single cloned insert, i.e., a putative promoter element, one can use the exonuclease III (ExoIII) and S1 or mung-bean nuclease method to generate sequential deletion mutants of the 3' and...
Topics
- Chloramphenicol O-Acetyltransferase
- Chromosome Deletion
- Cloning, Molecular
- Escherichia coli
- Genetic Vectors
- HeLa Cells
- Humans
- Mutation
- Plasmids