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Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells

29 Jan 2025

Abstract excerpt

To meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary. The human cytomegalovirus (CMV) promoter is widely used for RTP expression in CHO cells. To further improve RTP production, we truncated the human CMV intron and further evaluated the effect of four synthetic introns, including ctEF-1α...

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PMID: 39881196
DOI: 10.1038/s41598-025-87941-5

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