Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously

Abstract excerpt

A procedure for precise assembly of linear DNA constructs as long as 20 kb is proposed. The method, which we call long multiple fusion, has been used to assemble up to four fragments simultaneously (for a 10.8 kb final product), offering an additional improvement on the combination of long PCR and overlap extension PCR. The method is based on Pfu polymerase mix, which has a proofreading activity. We successfully...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to RNA and protein synthesis mechanisms
• Share to Viral Infections and Immunology Research
• Share to Viral Infectious Diseases and Gene Expression in Insects